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Four pale hands suggestively wearing suits as their sleeves, hold small amounts of US dollar bills.

Crispr Therapeutics Inc. stumbled in its initial public offering, more evidence that investors are cooling on the promising gene-editing technology known as CRISPR-Cas9.

One of three biotech companies using the technology locally to develop cures for genetic diseases, Crispr raised $56 million Tuesday in an IPO after the cutting the price and the number of shares offered. The stock closed essentially flat Wednesday on the first day of trading.

A pair of Cambridge-based rivals raised more capital in IPOs earlier this year. Editas Medicine, the first of the three to tap the public markets, raised $94 million in its IPO in February. Intellia Therapeutics Inc. raised $104 million in a May offering. But the shares of both companies have lost more than half their value from their opening-day prices.

“Timing is everything,” said Jim Weiss, senior portfolio manager at Chicago investment firm Segall Bryant & Hamill. “When you see a third IPO in the same space, there could be a perception by investors that this one is less attractive. At first people are gushing over the concept because it’s so...