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MINNEAPOLIS — For 14 years, Isaac Owusu’s faraway boys have tugged at his heart. They sent report cards from his hometown in Ghana and painstaking letters in fledgling English while he scrimped and saved to bring them here one day.

So when he became an American citizen and officials suggested taking a DNA test to prove his relationship to his four sons, he embraced the notion. Imagine, he marveled as a lab technician rubbed the inside of his cheek, a tiny swab of cotton would reunite his family.

But modern-day science often unearths secrets long buried. When the DNA results landed on Isaac Owusu’s dinner table here last year, they showed that only one of the four boys — the oldest — was his biological child.

Federal officials are increasingly turning to genetic testing to verify the biological bonds between new citizens and the overseas relatives they hope to bring here, particularly those from war-torn or developing countries where identity documents can be scarce or doctored.

But while the tests often lead to joyful reunions among immigrant families, they are...